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mouse a fetoprotein quantikine elisa kit  (R&D Systems)


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    R&D Systems mouse a fetoprotein quantikine elisa kit
    Mouse A Fetoprotein Quantikine Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+afp+quantikine+elisa+kit/pmc13068069-473-8-13?v=R%26D+Systems
    Average 94 stars, based on 35 article reviews
    mouse a fetoprotein quantikine elisa kit - by Bioz Stars, 2026-08
    94/100 stars

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    mouse a fetoprotein quantikine elisa kit  (R&D Systems)
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    ( A ) Reduced total body weight and increased liver to body weight ratio in PEX1-G844D mutants relative to littermate controls (N=4-6) at all age groups. 1 point represents 1 mouse. ( B ) Hematoxylin and eosin stain (H&E) showed hepatocyte enlargement with prominent nucleoli and ductular reaction (proliferating bile duct with lymphocyte infiltrates, arrows) at 1 month, foamy cell change (2 months), cluster cell-death (6 months), hepatitis (8 months), nodular hyperplasia (dashed line; 9 month), dilated sinusoid with congestion in hepatic tumor (12 months) and distinctive cellular atypia in tumor (15 months) (N=4-6). Top panel: 40x magnification. Bottom panel: 10x magnification. ( C, D ) Immunohistochemistry (IHC) of glutamate synthetase showed diffused positivity in PEX1-G844D mouse liver at age 12 months; glypican-3, a marker of hepatocellular carcinoma (HCC) was present at age 17 months. (E, F ) IHC for the proliferation marker, Ki67, showed increased number of hepatocytes with Ki67-positive nucleus (red arrows) in PEX1-G844D mutants compared to littermate control at age 2 months, 3-4 months, 5-6 months and 7-8 months (N=3-6). Representative images are shown at age 3, 6 and 8 months. Unpaired student t-test. ** P<0.01; *** P<0.001. ( G ) Serum alpha-fetoprotein <t>(AFP)</t> level was measured across age groups by <t>ELISA.</t> Chronic elevation was observed, with a spike at age 17-18 months. Unpaired student t-test. * P<0.05; ** P<0.01; *** P<0.001.
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    ( A ) Reduced total body weight and increased liver to body weight ratio in PEX1-G844D mutants relative to littermate controls (N=4-6) at all age groups. 1 point represents 1 mouse. ( B ) Hematoxylin and eosin stain (H&E) showed hepatocyte enlargement with prominent nucleoli and ductular reaction (proliferating bile duct with lymphocyte infiltrates, arrows) at 1 month, foamy cell change (2 months), cluster cell-death (6 months), hepatitis (8 months), nodular hyperplasia (dashed line; 9 month), dilated sinusoid with congestion in hepatic tumor (12 months) and distinctive cellular atypia in tumor (15 months) (N=4-6). Top panel: 40x magnification. Bottom panel: 10x magnification. ( C, D ) Immunohistochemistry (IHC) of glutamate synthetase showed diffused positivity in PEX1-G844D mouse liver at age 12 months; glypican-3, a marker of hepatocellular carcinoma (HCC) was present at age 17 months. (E, F ) IHC for the proliferation marker, Ki67, showed increased number of hepatocytes with Ki67-positive nucleus (red arrows) in PEX1-G844D mutants compared to littermate control at age 2 months, 3-4 months, 5-6 months and 7-8 months (N=3-6). Representative images are shown at age 3, 6 and 8 months. Unpaired student t-test. ** P<0.01; *** P<0.001. ( G ) Serum alpha-fetoprotein <t>(AFP)</t> level was measured across age groups by <t>ELISA.</t> Chronic elevation was observed, with a spike at age 17-18 months. Unpaired student t-test. * P<0.05; ** P<0.01; *** P<0.001.
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    Image Search Results


    ( A ) Reduced total body weight and increased liver to body weight ratio in PEX1-G844D mutants relative to littermate controls (N=4-6) at all age groups. 1 point represents 1 mouse. ( B ) Hematoxylin and eosin stain (H&E) showed hepatocyte enlargement with prominent nucleoli and ductular reaction (proliferating bile duct with lymphocyte infiltrates, arrows) at 1 month, foamy cell change (2 months), cluster cell-death (6 months), hepatitis (8 months), nodular hyperplasia (dashed line; 9 month), dilated sinusoid with congestion in hepatic tumor (12 months) and distinctive cellular atypia in tumor (15 months) (N=4-6). Top panel: 40x magnification. Bottom panel: 10x magnification. ( C, D ) Immunohistochemistry (IHC) of glutamate synthetase showed diffused positivity in PEX1-G844D mouse liver at age 12 months; glypican-3, a marker of hepatocellular carcinoma (HCC) was present at age 17 months. (E, F ) IHC for the proliferation marker, Ki67, showed increased number of hepatocytes with Ki67-positive nucleus (red arrows) in PEX1-G844D mutants compared to littermate control at age 2 months, 3-4 months, 5-6 months and 7-8 months (N=3-6). Representative images are shown at age 3, 6 and 8 months. Unpaired student t-test. ** P<0.01; *** P<0.001. ( G ) Serum alpha-fetoprotein (AFP) level was measured across age groups by ELISA. Chronic elevation was observed, with a spike at age 17-18 months. Unpaired student t-test. * P<0.05; ** P<0.01; *** P<0.001.

    Journal: bioRxiv

    Article Title: Longitudinal study of liver disease progression in the PEX1-Gly844Asp mouse model of mild Zellweger Spectrum Disorder

    doi: 10.1101/2025.05.08.652960

    Figure Lengend Snippet: ( A ) Reduced total body weight and increased liver to body weight ratio in PEX1-G844D mutants relative to littermate controls (N=4-6) at all age groups. 1 point represents 1 mouse. ( B ) Hematoxylin and eosin stain (H&E) showed hepatocyte enlargement with prominent nucleoli and ductular reaction (proliferating bile duct with lymphocyte infiltrates, arrows) at 1 month, foamy cell change (2 months), cluster cell-death (6 months), hepatitis (8 months), nodular hyperplasia (dashed line; 9 month), dilated sinusoid with congestion in hepatic tumor (12 months) and distinctive cellular atypia in tumor (15 months) (N=4-6). Top panel: 40x magnification. Bottom panel: 10x magnification. ( C, D ) Immunohistochemistry (IHC) of glutamate synthetase showed diffused positivity in PEX1-G844D mouse liver at age 12 months; glypican-3, a marker of hepatocellular carcinoma (HCC) was present at age 17 months. (E, F ) IHC for the proliferation marker, Ki67, showed increased number of hepatocytes with Ki67-positive nucleus (red arrows) in PEX1-G844D mutants compared to littermate control at age 2 months, 3-4 months, 5-6 months and 7-8 months (N=3-6). Representative images are shown at age 3, 6 and 8 months. Unpaired student t-test. ** P<0.01; *** P<0.001. ( G ) Serum alpha-fetoprotein (AFP) level was measured across age groups by ELISA. Chronic elevation was observed, with a spike at age 17-18 months. Unpaired student t-test. * P<0.05; ** P<0.01; *** P<0.001.

    Article Snippet: Serum levels of alpha-fetoprotein (AFP) and insulin were measured by solid phase sandwich ELISA: Mouse AFP Quantikine ELISA Kit (R&D system, MAFP00) and Mouse Ultrasensitive Insulin ELISA kit (ALPCO, #80-INSMSU-E01).

    Techniques: H&E Stain, Immunohistochemistry, Marker, Control, Enzyme-linked Immunosorbent Assay

    ( A ) ELISA quantification of serum insulin showed hypoglycemia in PEX1-G844D mutant mice relative to littermate controls at age 2.5 months in both fasted and fed ad-libitum state (N=6 per genotype). ( B, C ) Immunoblots quantified by densitometry revealed lower levels of proteins involved in hepatic de novo lipogenesis (SREBP1, FASN), glycolysis (PKLR) and glycogenolysis (G6PC), and increased amounts of proteins involved in hepatic lipid/lipoprotein uptake (VLDLR and CD36), lipoprotein breakdown (LPL) and fatty acid oxidation (HADHA) in liver of PEX-G844D mutants compared to littermate controls at age 2.5 months (N=6 per genotype). Unpaired student t-test. * P<0.05; ** P<0.01; *** P<0.001; ns: not significant.

    Journal: bioRxiv

    Article Title: Longitudinal study of liver disease progression in the PEX1-Gly844Asp mouse model of mild Zellweger Spectrum Disorder

    doi: 10.1101/2025.05.08.652960

    Figure Lengend Snippet: ( A ) ELISA quantification of serum insulin showed hypoglycemia in PEX1-G844D mutant mice relative to littermate controls at age 2.5 months in both fasted and fed ad-libitum state (N=6 per genotype). ( B, C ) Immunoblots quantified by densitometry revealed lower levels of proteins involved in hepatic de novo lipogenesis (SREBP1, FASN), glycolysis (PKLR) and glycogenolysis (G6PC), and increased amounts of proteins involved in hepatic lipid/lipoprotein uptake (VLDLR and CD36), lipoprotein breakdown (LPL) and fatty acid oxidation (HADHA) in liver of PEX-G844D mutants compared to littermate controls at age 2.5 months (N=6 per genotype). Unpaired student t-test. * P<0.05; ** P<0.01; *** P<0.001; ns: not significant.

    Article Snippet: Serum levels of alpha-fetoprotein (AFP) and insulin were measured by solid phase sandwich ELISA: Mouse AFP Quantikine ELISA Kit (R&D system, MAFP00) and Mouse Ultrasensitive Insulin ELISA kit (ALPCO, #80-INSMSU-E01).

    Techniques: Enzyme-linked Immunosorbent Assay, Mutagenesis, Western Blot